Plasmid
TDH3p-DmIa

Part:BBa_K4203001

Designed by: Yixuan Wang; Zongjun Lee   Group: iGEM22_BJWZ-China   (2022-10-12)


After codon optimization, those part is suitable for the fusion protein TDH3p-DmIav-sunTag-BFP expre

After codon optimization, the fusion protein TDH3p-DmIav-sunTag-BFP was planingto express in Saccharomyces cerevisiae BY4741. It was linked to the vector pESC-LEU by adding Sac I and Xho I restriction sites at the 5 'and 3' ends, respectively. 3352-4018 bp represents the TDH3 promoter, 4025-7393 bp represents DmIav component, 7394-7423 bp represents (GS) Χ5 linker, 7424-8137 bp represents sunTag, 8138-8194 bp represents P2A Linker, 8207-8914 bp represents TagBFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1116
    Illegal EcoRI site found at 5086
    Illegal XbaI site found at 12102
    Illegal PstI site found at 5325
    Illegal PstI site found at 6711
    Illegal PstI site found at 6717
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1116
    Illegal EcoRI site found at 5086
    Illegal NheI site found at 8931
    Illegal PstI site found at 5325
    Illegal PstI site found at 6711
    Illegal PstI site found at 6717
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1116
    Illegal EcoRI site found at 5086
    Illegal BglII site found at 6449
    Illegal BglII site found at 7280
    Illegal BglII site found at 7295
    Illegal XhoI site found at 8909
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1116
    Illegal EcoRI site found at 5086
    Illegal XbaI site found at 12102
    Illegal PstI site found at 5325
    Illegal PstI site found at 6711
    Illegal PstI site found at 6717
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1116
    Illegal EcoRI site found at 5086
    Illegal XbaI site found at 12102
    Illegal PstI site found at 5325
    Illegal PstI site found at 6711
    Illegal PstI site found at 6717
    Illegal NgoMIV site found at 2799
    Illegal AgeI site found at 1503
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5266
    Illegal BsaI.rc site found at 10272
    Illegal SapI site found at 9189
    Illegal SapI.rc site found at 7209


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Categories
Parameters
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